Me no chemist but to whoever doing a research on this field i think this will surely help A)Get a source for Claviceps Paspali B) L-tryptophane and succinamide have great effect on fermentation. The best composition of culture medium is 7% sorbitol, 3% succinamide, 0.1% L-tryptophane, 0.05 %MgSO47H2O, 0.03% KH2PO4 and pH 5.2 adjusted with ammonia. Through the cultivation of 14 days at 24-26 in shake flasks, the biggest content of the ergot alkali reaches 95.6 mg/L C) Large-scale production Obtain several ordinary 1 gallon jugs. Place a two-hole stopper in the necks of the jugs. Fit a short (6 inch) tube in one hole, leaving two inches above the stopper. Fit a short rubber tube to this. Fill a small (500 ml) Erlenmeyer flask with a dilute solution of sodium hypochlorite (NaClO). Extend a glass tube from the rubber so the end is immersed in the hypochlorite. Fit a long glass tube in the other stopper hole. It must reach near the bottom of the jug and have about two inches showing above the stopper. Attach a rubber tube to the glass tube and fit a short glass tube to the end of the rubber tube. Fill a large glass tube (1" x 6") with sterile cotton and fit one-hole stoppers in the ends. Fit the small glass tube in the end of the rubber tube into one stopper of the large tube. Fit another small glass tube into the other stopper. A rubber tube is connected to this and attached to small air pump (obtained from a tropical fish store). With this aeration equipment you can assure a supply of clean air to the Claviceps Paspali fungus while maintaining a sterile environment inside the solution. Dismantle the aerators. Place all the glass tubes, rubber tubes, stoppers and cotton in a paper bag, seal tightly with wire staples and sterilize in an autoclave. Fill the 1-gallon jugs 2/3 to 3/4 full with the culture medium and autoclave. While these things are being sterilized, homogenize in a blender the culture already obtained and use it to inoculate the material in the gallon jugs. The blender must be sterile. EVERYTHING must be sterile. Assemble the aerators. Start the pumps. A slow bubbling in each jug will provide enough oxygen to the cultures. A single pump may be connected to several filters. Let everything sit at room temperature (25 C) in a dark place (never expose ergot alkaloids to bright light - they will decompose) for a period of ten days. After ten days, adjust the culture to 1% ethanol using 95% ethanol under sterile conditions. Maintain growth for another two weeks. D) Extract ergot alkaloids After a total of 22 days growth period, the culture should be considered mature. Make the culture acidic with tartaric acid and homogenize in a blender for one hour. Adjust to pH 9 with ammonium hydroxide and extract with benzene or chloroform/iso-butanol mixture. Extract again with alcoholic tartaric acid and evaporate in a vacuum to dryness. The dry material is the salt (the tartaric acid salt, the tartrate) of the ergot alkaloids, and is stored in this form because the free basic material is too unstable and decomposes readily in the presence of light, heat, moisture, and air. To recover the free base for extraction of the amide or synthesis to LSD, make the tartrate basic with ammonia to pH 9, extract with chloroform, and evaporate in vacuo.