sorry missed out a part- Step II. Use Red light after you have run your lysergic acid through a column and are at the point of peptide coupling, you will want to switch to a red photographic safe light, which willbe used in any purification or further processes from this point onward. As always it is a good idea to simply let it stir in the dark.  2.80 grams of lysergic acid was added to 100 ml of magnetically stirring dichloromethane. Tothis was added 1.81 grams N,N -diethylmethylamine and the solution was allowed to stir forfive minutes. Then 5.70 grams of PyPOB was added and the solution was allowed to stir for anadditional five minutes. Then 0.84 grams of diethylamine was added and the reaction wasallowed to stir at RT(room temperature) for 60 minutes.The reaction mixture was quenched with 100 ml of 7.5M concentrated ammonium hydroxide,the layers were separated and the aqueous phase was then thrice extracted with 30 ml  dichloromethane, the organic layers were combined and rotary evaporated at 35C under highvacuum.The residue was dissolved in 40 ml of cold saturated sodium bicarbonate and extracted thricewith 20 ml ethyl acetate, the organic layers were combined and washed with deionized H 2 O,brine, and then dried over magnesium sulfate, filtered and rotary evaporated at 40C underhigh vacuum to a constant weight. Yield 3.13 grams before chromatography, 93%.Another run of 5.12 grams lysergic acid with the same amines, equivalents, and times, yielded 5.55 grams after chromatography, 9 all the best bro [/quote]